A Novel Nested Polymerase Chain Reaction (n-PCR) Assay for Identifying Sorghum nitidum

Diagnosis of canine coronavirus infection using nested-Polymerase Chain Reaction (n-PCR) by A. Pratelli; P0128.0899

Canine coronavirus (CCV) is a single-stranded RNA virus belonging to the Coronaviridae family and is responsible for generally mild gastroenteritis in pups. Infected dogs shed CCV in feces for 6-9 days, but shedding can be prolonged in some pups. The virus content of feces is very high at the time of clinical signs. Electron microscopic (EM) examinations of fecal suspensions or isolation in tis...

Polymerase Chain Reaction (PCR)

The polymerase chain reaction (PCR) is a powerful and widely used technique that has greatly advanced our ability to analyze genes. Genomic deoxyribonucleic acid (DNA) present in cells contains many thousands of genes. This makes it difficult to isolate and analyze any individual gene. PCR allows specific DNA sequences, usually corresponding to genes or parts of genes, to be copied from genomic...

A Diagnostic Multiplex Polymerase Chain Reaction Assay for Ocular Herpetic Infections.

A novel polymerase chain reaction assay to detect Mycoplasma genitalium.

AIMS To design and validate a polymerase chain reaction (PCR) assay targeting the 16S rRNA gene of Mycoplasma genitalium. METHODS Primers were designed that were complementary to the 16S rRNA gene sequence of M genitalium. After optimisation of the reaction conditions, the PCR was tested against nine M genitalium strains, a dilution series of M genitalium DNA, and a panel of common microorgan...

Polymerase Chain Reaction (PCR): A Short Review

was awarded the Nobel prize in Chemistry along with Michael Smith for his work on PCR2. The PCR is commonly carried out in a reaction volume of 10-200 μl in small reaction tubes (0.2-0.5 ml volumes) in a thermal cycler. The thermal cycler heats and cools the reaction tubes to achieve the temperatures required at each step of the reaction. Many modern thermal cyclers make use of the Peltier effe...